genetic diversity of paecilomyces variotii isolates in kerman province, iran

in this study, the genetic diversity among paecilomyces variotti isolaes was investigated. sampling was performed from pistachio garden in seven geographical region of kerman province during 2011-2012. seventy isolates were identified as p. variotii species using morphological criteria. the genetic diversity among 20 selected isolates was assayed by variation at ssr marker bands using 12 pair primers. the extracted genomic dna was amplified with 7 pair primers and pcr products were separated on an electrophoresis agarose gel. a total of 24 alleles were identified with an average of 3 alleles per primer combination. the mean of nei's coefficient among population was 0.62. the average of polymorphic information contents (pic) for the p. variotti ssr markers was 0.60. genetic relationships among isolates were represented by a dendrogram based on similarity coefficient matrix with upgma method. cluster analysis grouped the isolates into 5 major groups and a wide range of diversity across the tested isolates was found. the most distance was detected between zarand (var1) and rafsenjan (var15) isolates. these finding show that there are a week relation between subgrouped isolates and sampling regions. based on these results, we could conclude that ssr markers are suitable for illustrating levels of genetic diversity inward a population but not alone adequate for demonstrate a population structures and their related geographical regions.